Small 2014; 10(21):4386-4394

Reduced Aggregation and Cytotoxicity of Amyloid Peptides by Graphene Oxide/Gold Nanocomposites Prepared by Pulsed Laser Ablation in Water

Jingying Li1,[-],Qiusen Han1,[-], Xinhuan Wang1, Ning Yu2, Lin Yang1, Rong Yang1,*, Chen Wang1,*

 

1 CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China

2 Chinese PLA General Hospital, Beijing 100853, China

* Corresponding authors: E-mail: yangr@nanoctr.cn, wangch@nanoctr.cn

[-] These authors contributed equally to this work.

 

Abstract

We report a novel and convenient method to synthesize the nanocomposites combining graphene oxides (GO) with gold nanoparticles (AuNPs) and demonstrate their applications to modulate amyloid peptide aggregation. The nanocomposites produced by pulsed laser ablation (PLA) in water showed good biocompatibility and solubility. The reduced aggregation of amyloid peptides by the nanocomposites was confirmed by Thioflavin T fluorescence and atomic force microscopy. The cell viability experiments revealed that the presence of the nanocomposites could significantly reduce the cytotoxicity of the amyloid peptides. Furthermore, the depolymerization of peptide fibrils and inhibition of their cellular cytotoxicity by GO/AuNPs was also observed. These observations suggest that the nanocomposites combining GO and AuNPs have a great potential for designing new therapeutic agents and are promising for future treatment of amyloid-related diseases.

KEYWORDS: aggregation; graphene oxides; nanocomposites; pulsed laser ablation; β-amyloid

PMID: 25059878

 

Supplement:

Protein misfolding and aggregation is a pathological hallmark of several neurodegenerative diseases including Alzheimer’s disease. The effect of nanomaterials on peptide fibrillogenesis is drawing great attention. Here the nanocomposites combining graphene oxides with gold nanoparticles have been synthesized by pulsed laser ablation (PLA) in water. PLA method is a green and convenient technique to prepare novel biocompatible nanomaterials. Thioflavin T binding assay study demonstrates the efficient inhibition of amyloid peptide fibrillation by GO/AuNPs. And the inhibition effect of GO/AuNPs is more pronounced than that of GO solution. The cell viability experiments reveal that the nanocomposites can greatly reduce the cytotoxicity of the amyloid peptides. Our results demonstrate the viability of utilizing nanomaterials as the modulator for regulating amyloid-β aggregation, which will have a great potential in future treatment of amyloid-related diseases.

 figure

Fig 1. The GO/Au nanocomposites have been successfully produced by pulsed laser ablation in water. Greatly reduced aggregation and cytotoxicity of amyloid-β peptides by GO/AuNPs have been observed.

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