Cancer 2013 July-2

 

PXR and NF-κB correlate with the inducing effects of IL-1β and TNF-α on ABCG2 expression in breast cancer cell lines.

Eur J Pharm Sci. 2012 Sep 29;47(2):474-80.

Malekshah OM, Lage H, Bahrami AR, Afshari JT, Behravan J.

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

In this study we aimed to evaluate PXR and ABCG2 gene expression patterns and NF-κB activity induced by proinflammatory cytokines in different breast normal and carcinoma cells. The effects of proinflammatory cytokines on ABCG2 and PXR mRNA expression were studied using real-time PCR. Western blot analysis used for evaluating the protein levels of ABCG2, PXR and the active form of NF-κB (p65 in nuclear protein extract). Significant inductions in the ABCG2 and PXR mRNA and protein levels and NF-κB activity, were observed in MCF7, BT-474, CAL51, 184A1 and HBL100 cells, upon treatment with 50 ng/ml of IL-1β and TNF-α. On the contrary significant reduction of the ABCG2 and PXR mRNA and protein levels and NF-κB activity, were observed in MDA-MB-435 cell line. In conclusion, IL-1β and TNF-α induced ABCG2 and PXR expression and NF-κB activity in some breast cancer and normal cell lines. Similar patterns of induction and reduction in PXR and ABCG2 genes and NF-κB activity suggest a probable relationship between ABCG2, PXR and NF-κB. Copyright © 2012 Elsevier B.V.

PMID: 22750628

Multiselect Ultimate Query Plugin by InoPlugs Web Design Vienna | Webdesign Wien and Juwelier SchönmannMultiselect Ultimate Query Plugin by InoPlugs Web Design Vienna | Webdesign Wien and Juwelier Schönmann