Int J Clin Exp Pathol. 2015 Nov;8(11):14965-70.

Association of MMP-2 expression and prognosis in osteosarcoma patients

 

Miao Zhang, Xuran Zhang.

Surgical Ward 3, Department of Orthopaedics, Fuxin Central Hospital, Fuxin, Liaoning Province, China 123000.

 

Abstract

OBJECTIVE: Our aim was to investigate the MMP-2 expression and its prognostic value in osteosarcoma patients.

METHODS: We performed RT-qPCR to detect the expression of MMP-2 in 45 paired osteosarcoma tissues and adjacent noncancerous tissues. Immunohistochemical staining assay was used to verify the expression of MMP-2 protein in osteosarcoma patients. Independent-sample T test was used to analyze the difference of MMP-2 expression level between osteosarcoma and control groups. The relationship between clinicopathologic factors and MMP-2 expression was analyzed by chi-square test. Kaplan-Meier was performed to analyze the association of MMP-2 expression and overall survival rate. The prognostic value of clinicopathologic factors and MMP-2 was estimated via Cox regression analysis.

RESULTS: RT-qPCR revealed that the expression of MMP-2 was up-regulated in osteosarcoma group compared with the control group. Besides MMP-2 expression was influenced by pulmonary metastasis (P<0.05) while gender, age, tumor site and Enneking stage showed no obvious impact (P>0.05). Kaplan-Meier curve revealed that patients with positive MMP-2 expression had a shorter survival time than those with negative MMP-2 expression, and the survival rates were 18.5% (5/27) and 44.4% (8/18), respectively. Cox regression analysis indicated that pulmonary metastasis and expression of MMP-2 gene were important factors in the prognosis of osteosarcoma.

CONCLUSION: The expression of MMP-2 was associated with pulmonary metastasis, and was related to the prognosis of osteosarcoma. MMP-2 could act as an independent prognostic marker in osteosarcoma patients.

PMID: 26823829

 

Additional information:

Osteosarcoma has been recognized as one of the most serious malignant tumors in children and adolescents, with a strong tendency of distant metastasis and low 5-year survival rate. Although without revolutionary breakthrough available in clinical treatment at the moment, we still hope to provide comprehensive medical care to osteosarcoma patients, such as accurate prognosis and improvement in quality of life, especially in prevention of pulmonary metastasis and effective pain management. This study showed that MMP-2 was an independent prognostic factor in osteosarcoma associated with pulmonary involvement and long-term survival rate. In addition, as MMP-2 is also associated with the onset and chronic status of bone cancer pain, we are going to further investigate whether the suppression of MMP-2 expression could inhibit tumor metastasis and alleviate cancer-induced bone pain in animal models of osteosarcoma.

Acknowledgements:

This study was supported by the Orthopaedic Pain Medicine Development Program of Fuxin Central Hospital.

 

Miao Zhang - PMID 26823829 - photograph of authorsContact:

Miao Zhang, M.D.

Director, Surgical Ward 3, Department of Orthopaedics, Fuxin Central Hospital, Fuxin, Liaoning Province, China 123000.

zhmiao523@yeah.com; zhangmiao197702@163.com

http://www.fxzxyy.com/newsShow.Asp?id=1368

Multiselect Ultimate Query Plugin by InoPlugs Web Design Vienna | Webdesign Wien and Juwelier SchönmannMultiselect Ultimate Query Plugin by InoPlugs Web Design Vienna | Webdesign Wien and Juwelier Schönmann