Consequences of the Combined alpha-tocopherol, Ascorbic Acid and alpha-lipoic Acid on the Glutathione, Cholesterol and Fatty Acid Composition in Muscle and Liver of Diabetic Rats.

Iran J Basic Med Sci.2013 Feb;16(2):165-172.

Okkes YILMAZ1, Yasemin ERSAN1, Ayse Dilek OZSAHIN*2, Ali Ihsan OZTURK3, Yusuf OZKAN4

1 Department of Biology, Faculty of Science, Firat University, 23119-Elazig, Turkey

2 Department of Biology, Faculty of Science, Bitlis Eren University, 13000-Bitlis, Turkey

3 Department of Chemistry, Faculty of Science, Harran University, 63100-S.Urfa, Turkey

4 Department of Internal Medicine, School of Medicine, Firat University, 23119-Elazig, Turkey



Objective(s): Our objective was to evaluate the effects of a triple antioxidant combination [α-tocopherol (AT), ascorbic acid (AA) and α-lipoic acid (LA); AT+AA+LA] on the cholesterol and glutathione levels, and the fatty acid composition of liver and muscle tissues in diabetic rats. Materials and Methods: Forty-three Wistar rats were randomly divided into five groups. The first group was used as a control. The second, third and fourth groups received STZ (45 mg/kg) in citrate buffer. The fourth and fifth groups were injected with intraperitoneal (IP) 50 mg/kg DL-AT and 50 mg /kg DL-LA four times per week and received watersoluble vitamin C (50 mg/kg) in their drinking water for a period of six weeks. Results: Liver cholesterol levels in the AT+AA+LA group were lower than the control (P<0.05). Glutathione level was lower in D-2 (P<0.05) and were higher in D+AT+AA+LA and AT+AA+LA groups than the control groups (P≤ 0.05). The muscle cholesterol levels in the D-1 and D+AT+AA+LA groups were higher than the control group (P≤ 0.05). The levels of oleic acid were higher in the D-1 group and lower in the D-2 group (P<0.001). The arachidonic acid level in the D-1 and D-2 groups were lower (P<0.05), and higher in the D+AT+AA+LA group. Conclusion: Our results revealed that glutathione levels and the Stearoyl CoA Desaturase enzyme products in liver tissues of diabetic and non-diabetic rats were increased by triple antioxidant mixture.

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