Diabetes 2013 May-26

 

Morphophisiological evaluation of a kind of propolis in tissue repair in diabetic rats.

Lat Am J Pharm.2012 Jun;31(5):777-781.

Mezadri, Telmo. J; Tames, David R.; Reis, Renata K.T.; Ortolan, Xana R.

Abstract

The objective of this work was to study the effects of green propolis on skin wounds in diabetic rats. We used 10 animals, creating two wounds of 5 mm in diameter on the dorsal region, using a punch. The left wounds were treated topically with Brazilian green propolis (5 %) while the right wounds received nothing. The tissue repair was evaluated in microscopy after 72 h. The results showed similar numbers of leukocytes and macrophages, however the number of fibroblasts, cell density and fibrillogenesis were increased in the experimental group, indicating the action of propolis on the skin wounds.

 

Supplement:

Baccharis dracunculifolia is the raw material used in the production of green propolis. It has various biological properties, such as anti-inflammatory, wound healing, immunomodulatory, anticarcinogenic, antioxidant, antibacterial, antiparasitic and antiviral properties, and is the subject of many studies. The purpose of this study was to evaluate the effects of topical treatment with propolis on the repair of skin wounds in diabetic rats. Ten animals were used, inducing the diabetes by administering an aqueous solution of streptozotocin (Sigma Aldrich). We made two wounds of 5 mm in diameter on the dorsal region, using a punch. The left wounds were treated topically with Brazilian green propolis (5 %) while the right wounds received nothing. The tissue repair was evaluated in microscopy after 72 h.

The results for the leukocytes, macrophages and fibroblasts count, comparing the treated wounds and the controls in the first 72 h, are shown in Table 1. The mean of leukocyte and macrophages in the wounds treated with propolis and untreated shows no significant difference between the groups. Regarding the fibroblast count, the mean values in treated wounds and control showed a statistically significant increase of this cell type in the propolis group, indicating greater activity in the synthesis of extracellular matrix components.

 

Telmo José  Mezadri

Table 1. Average leukocytes, macrophages and fibroblasts count, comparing treated and control injuries in the first 72 h. The wounds treated with propolis showed a significantly increased of number of fibroblasts (p <0.005 – ANOVA post hoc Tukey test).

Studies support the evidence that in diabetes there is a dysfunction of fibroblasts, with a prolonged inflammatory phase and a decrease in the biosynthesis of collagen. Staak Júnior et al.(2011) evaluating the topical effect of propolis on the proliferation of fibroblasts in healing by secondary intention, also showed a significant increase in the number of these cells in the experimental group compared to the control group, specially between days 4 and 7 of  treatment. In our study it was observed that the wounds treated with propolis 5 % had a significantly higher number of fibroblasts, with signs of fibrillogenesis, suggesting increased fibroblastic activity and collagen synthesis compared with untreated wounds, which may be related to an induction in the synthesis of fibroblast growth factors, as suggested by Moura (2004).

The results showed similar numbers of leukocytes and macrophages; however the number of fibroblasts, cell density and fibrillogenesis were increased in the experimental group, indicating the action of propolis on skin wounds.

 

Staak Júnior, M.C, P.H. Colchon, A.G. Portes, T.J. Mezadri, D.R. Tames & S.F. Andrade

(2011) Lat. Am. J. Pharm. 30: 383-7.

 Moura, S.A. (2004) Desenvolvimento de modelo experimental para estudo do processo de cicatrização por segunda intenção de feridas cutâneas em coelhos, utilizando extratos da própolis verde de Minas Gerais – Brasil. Dissertação (Mestrado em Medicina Veterinária) – Universidade Federal de Viçosa. Brazil.

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