Structural and biochemical characteristics of arterial stiffness in patients with atherosclerosis and in healthy subjects.

Hypertens Res. 2012 Oct;35(10):1032-7.

Zagura M, Kals J, Serg M, Kampus P, Zilmer M, Jakobson M, Unt E, Lieberg J, Eha J.

Department of Biochemistry, Centre of Excellence for Translational Medicine, University of Tartu, Tartu, Estonia. Maksim.Zagura@kliinikum.ee

Abstract

Arterial stiffness is an independent predictor of vascular morbidity and mortality in patients with atherosclerosis. Angiographic score (ASc) reflects severity of atherosclerosis in patients with peripheral arterial disease (PAD). Osteopontin (OPN) and oxidized low-density lipoprotein (oxLDL) are involved in the pathogenesis of atherosclerosis. The aim of the present study was to evaluate the association between arterial stiffness, ASc, serum OPN and oxLDL in patients with symptomatic PAD, and in clinically healthy subjects. We studied 79 men with symptomatic PAD (mean age 64±7 years) and 84 healthy men (mean age 63±8 years). Calculation of the ASc was based on severity and location of atherosclerotic lesions in the arteries of the lower extremities. Aortic pulse wave velocity (aPWV) was evaluated by applanation tonometry using the Sphygmocor device. Serum OPN and oxLDL levels were determined by enzyme-linked immunosorbent assay. The aPWV (10±2.4 vs. 8.4±1.7 (m s(-1)); P<0.001), OPN (75 (62.3-85.8) vs. 54.8 (47.7-67.9) (ng ml(-1)); P<0.001) and oxLDL (67 (52.5-93.5) vs. 47.5 (37-65.5); P<0.001) were different for the patients and for the controls. In multiple regression models, aPWV was independently determined by ASc, log-OPN, log-oxLDL and estimated glomerular filtration rate in the patients (R2=0.44; P<0.001) and by log-OPN, log-oxLDL, age and heart rate in the controls (R2=0.38; P<0.001). The independent relationship of a PWV with serum levels of OPN and oxLDL in the patients with PAD and in the controls indicates that OPN and oxLDL might influence arterial stiffening in patients with atherosclerosis and in clinically healthy subjects.

PMID: 22739422

 

Supplement:

Hypertens Res (Figure 1)

Figure 1. Correlation between aPWV and ASc (r = 0.37, P = 0.001) for the PAD patients.

 Hypertens Res (Figure 2)

Figure 2. Correlation between aPWV and log-OPN for the patients (r = 0.39, p < 0.001) and for the control subjects (r = 0.41, p < 0.001). Filled dots represent patients; empty dots represent controls. Continuous line represents regression line through the patient data; interrupted line represents regression line through the control data.

 

 

Hypertens Res (Figure 3)

Figure 3. Relationship between aPWV and the tertiles of OPN and oxLDL in PAD patients. aPWV, aortic pulse wave velocity; MAP, mean arterial pressure; OPN, osteopontin; oxLDL, oxidized low-density lipoprotein.

 

 

Hypertens Res (Figure 4)

Figure 4. Relationship between aPWV and the tertiles of OPN and oxLDL in controls. aPWV, aortic pulse wave velocity; MAP, mean arterial pressure; OPN, osteopontin; oxLDL, oxidized low-density lipoprotein.

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