Biofouling. 2015 Aug;31(7):543-54.

Comparative studies of the immunogenicity and protective potential of biofilm vs planktonic Staphylococcus aureus vaccine against bovine mastitis using non-invasive mouse mastitis as a model system.

 

Gogoi-Tiwari J, Williams V, Waryah CB, Eto KY, Tau M, Costantino P, Tiwari HK, Mukkur T.

Faculty of Health Sciences, School of Biomedical Sciences, Curtin Health Innovation Research Institute , Curtin University , Bentley, Perth , Australia.

 

Abstract

This study was undertaken to compare the immunogenicity and protective potential of biofilm vs planktonic Staphylococcus aureus vaccine for the prevention of mastitis using the mouse as a model system. Mice immunized with formalin-killed whole cell vaccine of S. aureus residing in a biofilm when delivered via an intramammary route produced a cell mediated immune response. Mice immunized with this biofilm vaccine showed significant reductions in colonization by S. aureus in mammary glands, severity of clinical symptoms and tissue damage in mammary glands in comparison with the mice immunized with formalin-killed whole cells of planktonic S. aureus. The planktonic vaccine administered by a subcutaneous route produced a significantly higher humoral immune response (IgG1 and IgG) than the biofilm vaccine. However, considering the host response, tissue damage, the clinical severity and colonization of S. aureus in mammary glands, the biofilm vaccine performed better in immunogenicity and protective potential when administered by the intramammary route.

KEYWORDS: Staphylococcus aureus; biofilm; mouse mastitis model; planktonic; vaccine

PMID: 26293793

 

Summary

Over the last 2 decades, great deal of emphases has been placed to find ways to prevent the emergence of antimicrobial resistance of Staphyloccoccus aureus and enforcement of universal precautions in the hospitals, where possible, that has resulted in a significant reduction of hospital acquired infections in patients. However, little attention has been paid to the strategy of developing effective vaccines as a means of reducing the potential burden of this pathogen particularly in animals on farms in which the use of antibiotics remains rampant. Given the emerging knowledge on the transmission of antibiotic-resistant S. aureus between animals and humans, and the need to develop effective but affordable S. aureus-associated bovine mastitis vaccines, the authors decided to evaluate the immunogenicity and protective potential of biofilm versus planktonic Staphylococcus aureus whole cell vaccines for the prevention of mastitis using mouse as a model system. Mice immunized with formalin killed whole cell of S. aureus residing in biofilm and delivered via i/mam route produced not only antibodies but also cell-mediated immune response (CMI). Mice immunised with the killed whole cell biofilm vaccine by the intramammary (i/mam) route significantly reduced bacterial burden of S. aureus in mammary gland, severity of clinical symptoms and tissue damage in mammary gland in comparison with the mice immunized with the killed whole cell of planktonic S. aureus vaccine The antibody isotypes induced by the biofilm vaccine were both IgG1 and IgG2a (indirect indicator of CMI) whereas subjects vaccinated with the planktonic vaccine produced mainly IgG1 isotype. Mice vaccinated with the biofilm vaccine by the i/mam route also produced significant levels interferon gamma indicating induction of CMI whereas those vaccinated with the planktonic vaccine did not. Considering the host response, tissue damage, clinical severity and colonisation of S. aureus in mammary gland, the biofilm vaccine performed better in immunogenicity and protective potential than the planktonic vaccine when administered by i/mam route than the subcutaneous route.

 

 

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