Sequence-specific and visual identification of the influenza virus NS gene by azobenzene-tethered bis-peptide nucleic acid.

PLoS One. 2013 May 21;8(5):e64017.

Kaihatsu K, Sawada S, Nakamura S, Nakaya T, Yasunaga T, Kato N.

The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.

Abstract

To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.

PMID: 23704970

Kunihiro Kaihatsu-1

Contact:

Dr. Kunihiro Kaihatsu

email: kunihiro@sanken.osaka-u.ac.jp

 

 

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