Acid aspiration provokes the pneumonia caused by multidrug-resistant Acinetobacter baumannii in BALB/c mice.

Int J Infect Dis. 2013 Jun;17(6):e454-60.

Chiang SR, Tang HJ, Chen CH, Chen CC, Lee WY, Chang PC, Chiu YH, Chuang YC.

Department of Internal Medicine, Chi Mei Medical Center, 901, Chung-Hwa Road, Yung-Kang Dist., Tainan City, Taiwan.

 

Abstract

OBJECTIVE: To determine whether acid aspiration provokes the development of multidrug-resistant Acinetobacter baumannii (MDRAB) pneumonia in its host.

METHODS: Groups of mice were inoculated intratracheally (IT) with 50 μl of 0.1N HCl and 1 × 10(8) colony-forming units (CFU) Ab396 (A+Ab group), or 50 μl of 0.1N HCl and 20 μl of 0.9% saline (A+S group), or 20 μl of 0.9% saline and 1 × 10(8) CFU of Ab396 (S+Ab group), or 50 μl of 0.9% saline and 20 μl of 0.9% saline (S+S group). Cytokines, bacterial loads in the bronchoalveolar lavage fluid (BALF), lung permeability, histopathology of the lungs, and survival rates were evaluated.

RESULTS: Only the A+Ab mice developed extensive Ab396 pneumonia and had significantly elevated bacterial loads, increased lung leakage, and lower levels of tumor necrosis factor alpha (TNF-α) compared with the other three groups (p<0.05, Mann-Whitney U-test). Moreover, a strong synergistic effect (p<0.05, two-way analysis of variance) was observed between the acid induction and Ab396 infection, resulting in lung injury and an unfavorable survival outcome.

CONCLUSIONS: Lung injury caused by acid aspiration provoked secondary MDRAB pneumonia; also synergistic effects between acid aspiration and Ab396 infection resulted in a detrimental outcome in the infected mice.

Copyright © 2013 International Society for Infectious Diseases.

PMID: 23522637

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