J Clin Microbiol. 2014 Apr;52(4):1269-73. doi: 10.1128/JCM.03346-13.

Bloodstream infections caused by Pseudomonas spp.: how to detect carbapenemase producers directly from blood cultures.

Dortet L, Boulanger A, Poirel L, Nordmann P.

INSERM U914 “Emerging Resistance to Antibiotics,” South-Paris Medical School, K.-BicĂȘtre, France.

 

Abstract

The Carba NP test has been evaluated to detect carbapenemase-producing Pseudomonas spp. directly from blood cultures. This rapid and cost-effective test permits an early identification of carbapenemase-producing Pseudomonas spp. directly from blood cultures with excellent sensitivity and specificity. Results may be useful in particular for guiding the first-line therapy and epidemiological purposes.

PMID: 24501031

 

SUPPLEMENT:

The Carba NP tested performed directly from positive blood cultures is aimed to detect any type of carbapenemase producers in P. aeruginosa. The most frequent carbapenemases are carbapenemases of the VIM, IMP and NDM types. This test will differentiate carbapenem-resistant isolates that produce a carbapenemase that are transmissible resistance marker from those that are carbapenem-resistant due to other mechanisms mostly due to outer-membrane OprD permeability defect. That latter mechanism of resistance is the most frequent but it is not transmissible.

Suppl-FiG-1Fig 1. Antibiotic susceptibility performed by disk diffusion of a Pseudomonas aeruginosa isolate that produces the most frequent carbapenemase in P. aeruginosa, i.e. VIM-2

 

 

Suppl-Fig-2-j
Fig 2. Schematic representation of the CarbaNP test for detection of carbapenemase producers in P. aeruginosa from bloodstream infections

 

Multiselect Ultimate Query Plugin by InoPlugs Web Design Vienna | Webdesign Wien and Juwelier SchönmannMultiselect Ultimate Query Plugin by InoPlugs Web Design Vienna | Webdesign Wien and Juwelier Schönmann