Cell Transplant. 2014 Feb;23(2):167-79.

Autologous G-CSF-mobilized peripheral blood CD34+ cell therapy for diabetic patients with chronic nonhealing ulcer.

Tanaka R, Masuda H, Kato S, Imagawa K, Kanabuchi K, Nakashioya C, Yoshiba F, Fukui T, Ito R, Kobori M, Wada M, Asahara T, Miyasaka M.

Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan.

 

ABSTRACT:

Recently, animal studies have demonstrated the efficacy of endothelial progenitor cell (EPC) therapy for diabetic wound healing. Based on these preclinical studies, we performed a prospective clinical trial phase I/IIa study of autologous G-CSF-mobilized peripheral blood (PB) CD34(+) cell transplantation for nonhealing diabetic foot patients. Diabetic patients with nonhealing foot ulcers were treated with 2 × 10(7) cells of G-CSF-mobilized PB CD34(+) cells as EPC-enriched population. Safety and efficacy (wound closure and vascular perfusion) were evaluated 12 weeks posttherapy and further followed for complete wound closure and recurrence. A total of five patients were enrolled. Although minor amputation and recurrence were seen in three out of five patients, no death, other serious adverse events, or major amputation was seen following transplantation. Complete wound closure was observed at an average of 18 weeks with increased vascular perfusion in all patients. The outcomes of this prospective clinical study indicate the safety and feasibility of CD34(+) cell therapy in patients with diabetic nonhealing wounds.

PMID: 23107450

 

Reference:

  1. Tanaka R, Masuda H, Kato S, Imagawa K, Kanabuchi K, Nakashioya C, Yoshiba F, Fukui T, Kobori M, Wada M, Asahara T, Miyasaka M. Autologous G-CSF mobilized peripheral blood CD34+ cell therapy for diabetic patients with chronic non-healing ulcer. Cell Transplant. 2014;23(2):167-79
  2. Tanaka R, Vaynrub M, Masuda H, Ito R, Kobori M, Miyasaka M, Mizuno H, Warren SM, Asahara T. Quality-control culture system restores diabetic endothelial progenitor cell vasculogenesis and accelerates wound closure. Diabetes. 2013;62:3207-3217
  3. Tanaka R, Wada M, Kwon S, Masuda H, Carr J, Ito R, Miyasaka M, Warren SM, Asahara T, Tepper OM. The Effects of Flap Ischemia on Normal and Diabetic Progenitor Cell Funnction. Plast Reconstr Surg.121(6).1929-42.2008.

 

Contact Information:

Rica Tanaka M.D., PhD
Associate Professor
Juntendo University Department of Plastic and Reconstructive Surgery
rtanaka@juntendo.ac.jp

 

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