Int J Med Sci. 2015 Jan 8;12(2):154-62. doi: 10.7150/ijms.9964.

New adipose tissue formation by human adipose-derived stem cells with hyaluronic acid gel in immunodeficient mice.

Huang SH1, Lin YN2, Lee SS3, Chai CY4, Chang HW5, Lin TM2, Lai CS3, Lin SD3.
  • 11. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan ; 2. Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan ; 3. Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan ; 4. Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
  • 23. Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
  • 32. Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan ; 3. Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan ; 4. Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
  • 45. Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
  • 56. Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan.

 

Abstract

BACKGROUND: Currently available injectable fillers have demonstrated limited durability. This report proposes the in vitro culture of human adipose-derived stem cells (hASCs) on hyaluronic acid (HA) gel for in vivo growth of de novo adipose tissue.

METHODS: For in vitro studies, hASCs were isolated from human adipose tissue and were confirmed by multi-lineage differentiation and flow cytometry. hASCs were cultured on HA gel. The effectiveness of cell attachment and proliferation on HA gel was surveyed by inverted light microscopy. For in vivo studies, HA gel containing hASCs, hASCs without HA gel, HA gel alone were allocated and subcutaneously injected into the subcutaneous pocket in the back of nude mice (n=6) in each group. At eight weeks post-injection, the implants were harvested for histological examination by hematoxylin and eosin (H&E) stain, Oil-Red O stain and immunohistochemical staining. The human-specific Alu gene was examined.

RESULTS: hASCs were well attachment and proliferation on the HA gel. In vivo grafts showed well-organized new adipose tissue on the HA gel by histologic examination and Oil-Red O stain. Analysis of neo-adipose tissues by PCR revealed the presence of the Alu gene. This study demonstrated not only the successful culture of hASCs on HA gel, but also their full proliferation and differentiation into adipose tissue.

CONCLUSIONS: The efficacy of injected filler could be permanent since the reduction of the volume of the HA gel after bioabsorption could be replaced by new adipose tissue generated by hASCs. This is a promising approach for developing long lasting soft tissue filler.

KEYWORDS: adipose tissue; human adipose-derived stem cells

PMID: 25589892

 

SUPPLEMENTS:

Inverted light microscope image showing hASCs seeding in HA gel formed microspheres, these microspheres is easily for injection to the subcutaneously in the back of mice. This concept may apply to clinical use such as various soft tissue filler with cell assisted or HA gel mixed with cartilage cells to inject in to cartilage damaged joint.

fig1

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