J Buon.2013 Apr-Jun;18(2):504-510

GE132+Natural: Novel promising dietetic supplement with antiproliferative influence on prostate, colon, and breast cancer cells.

Ivana Okić Đorđević1, Drenka Trivanović1, Jelena Krstić1, Aleksandra Jauković1, Slavko Mojsilović1, Juan Francisco Santibanez1, Milan Terzić2,3, Dušan Vešović4, Diana Bugarski1

1 Laboratory for Experimental Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, Belgrade, Serbia

2  Clinic of  Obstetrics and Gynecology, Clinical Center of Serbia, Belgrade, Serbia

3   Faculty  of Medicine, University of Belgrade, Belgrade, Serbia

4  Health Care Center BEL MEDIC, Belgrade, Serbia



Purpose: Natural products have been investigated for promising new leads in pharmaceutical development. The purpose of this study was to analyze the biological effect of GE132+Natural, novel supplement consisting of five compounds: Resveratrol, Ganoderma lucidum, Sulforaphane, Lycopene and Royall jelly (RJ).

Methods: The antiproliferative activity of GE132+Natural was tested on three different human cancer cell lines: MCF7 (breast cancer cells), PC3 (prostate cancer cells), and SW480 (colon cancer cells), as well as on EA.hy 926, normal human endothelial cell line. Additionally, the cytotoxicity of GE132+Natural on the proliferation of primary human mesenchymal stem cells isolated from dental pulp (DP-MSC), along with its in vitro impact on different peripheral blood parameters, was determined.

Results: The results revealed high antiproliferative activity of GE132+Natural on all tested cancer lines (PC3, MCF7 and SW480), as well as on the EA.hy 926 endothelial cell line in a dose-dependent manner. However, applied in a wide range of concentrations GE132+Natural did not affect both the proliferation of primary mesenchymal stem cells and the peripheral blood cells counts.

Conclusions: The data obtained demonstrated that GE132+Natural is effective in inhibiting cancer cell proliferation, indicating its potential beneficial health effects. Additionally, results pointed that adult mesenchymal stem cells might be valuable as a test system for evaluating the toxicity and efficacy of new medicines or chemicals.



Natural products of strong medicinal values are gaining importance in cancer therapy and prevention, especially in the light of the serious side effects posed by chemically produced medicinal derivatives. Hence, it becomes pivotal to investigate other strategies to control tumor proliferation. In the present study, we evaluated the effects of dietary supplement GE132+Natural, on proliferation of three different human tumor cell lines including prostate cancer cell line (PC-3), breast cancer cell line (MCF7), and colorectal adenocarcinoma cell line (SW-480), along with the effects on human endothelial EA.hy26 cell line and human primary cells – DP-MSCs.

The antitumoral activity of the GE132+Natural confirmed by the significant growth suppression of all cancer cell lines tested is in agreement with previous studies demonstrating the antitumorogenic effects of all components of the GE132+Natural supplement themselves [1,2,3,4,5].

When the antiproliferative effect of GE132+Natural extract on EA.hy 926 cells was evaluated, assuming that “hybrid” cell lines are often designated as normal cell lines and are used as control cells to prove the non-cytotoxic effects of various chemotherapeutic agents, the growth inhibition pattern obtained was in a way unexpected. However, immortalized cells usually show anomalous behavior and phenotype, which do not reflect the mechanisms observed in their normal homologous cells. Since the vascular endothelial cell proliferation is a key initial step in angiogenesis, the effect of GE132+Natural could be related to the involvement of the supplement components in this process [6,7].

To determine the cytotoxic effect of tested GE132+Natural, we measured the effect on mature blood cells, erythrocytes, leukocytes, and platelets, and our results indicated that GE132+Natural does not cause significant changes in various peripheral blood cell counts in vitro. However, more important and valuable are the data obtained with the human MSCs, in which GE132+Natural, in a wide range of concentrations, did not affect their proliferation and viability. The unique properties of MSCs, such as unlimited proliferation ability and plasticity to generate other cell types, along with various readily available sources of primary human cells, clearly identify their potential benefits in toxicology, although data demonstrating that these cells can confidently be used to perform in vitro acute toxicity tests are just starting to be reported [8].

In conclusion, our results demonstrated that GE132+Natural is beneficial in inhibiting cancer cell proliferation and indicated its potential antiangiogenic effects. Additionally, our data pointed that adult mesenchymal stem cells can confidently be used to perform in vitro acute toxicity tests. Indeed, compared to traditional in vitro systems based on transformed or immortalized cell lines, hMSCs provide a more accurate modeling of in vivo conditions and can determine efficacy of new medicines or chemicals along with toxicity.



1. Nguyen AV, Martinez M, Stamos MJ. Results of a phase I pilot clinical trial examining the effect of plant-derived resveratrol and grape powder on Wnt pathway target gene expression in colonic mucosa and colon cancer. Cancer management and rearch 2009;1:25–37.

2. Martínez-Montemayor MM, Acevedo RR, Otero-Franqui E, Cubano LA, Dharmawardhane SF. Ganoderma lucidum (Reishi) Inhibits Cancer Cell Growth and Expression of Key Molecules in Inflammatory Breast Cancer. Nutr Cancer 2011; 63: 1085–1094.

3. Seren S, Lieberman R, Bayraktar UD et al. Lycopene in Cancer Prevention and Treatment. Am J Ther. 2008;15(1):66-81.

4. Zhang Y, Kensler TW, Cho CG, Posner GH, Talalay P. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornyl isothiocyanates. Proc Natl Acad Sci USA1994;91(8):3147-3150.

5. Mishima S, Suzuki KM, Isohama Y, Kuratsu N, Araki Y, Inoue M, Miyata T. Royal jelly has estrogenic effects in vitro and in vivo. J Ethnopharm 2005; 101:215-220.

6. Izuta H, Shimazawa M, Tsuruma K et al. Bee products prevent VEGF-induced angiogenesis in human umbilical vein endothelial cells. BMC Complem Altern M 2009; 9:45.

7. Asakage M, TsunoNH, Kitayama J et al. Sulforaphane induces inhibition of human umbilical vein endothelial cells proliferation by apoptosis. Angiogenesis 2006;9:83–91.

8. Scanu M, Mancuso L, Cao G. Evaluation of the use of human Mesenchymal Stem Cells for acute toxicity tests. Toxicology in Vitro 2011;25:1989–1995.

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