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Oxidative Stress

 

  • Oxidative stress markers
  • ELISA kits
  • Monoclonal antibodies

JaICA

Elisas to detect oxidative stress markers for DNA oxidation, lipid oxidation, protein oxidation and antioxidants.

Biomed Frontiers made research into control of aging and sells many research products related to oxidative stress. We detect oxidative stress markers for DNA oxidation, lipid oxidation, protein oxidation and antioxidants.

The Genprice Biomed Frontiers monoclonal antibodies and ELISA kits detect products of oxidative damage caused by reactive oxygen species.

 

Type of
marker		 Item Spec. Applications Remark
Urine Serum Cells
DNA
oxidation
marker		 8-OHdG
New 8-OHdG Check ELISA
(Code. KOG-200SE), ELISA: 450nm, 96 wells

Highly Sensitive 8-OHdG Check ELISA
(Code. KOG-HS10E), ELISA: 450nm, 96 wells

Anti 8-OHdG monoclonal antibody (N45.1)
(Code: MOG-020P / MOG-100P)

  
Assay range:
0.5 - 200 ng/mL

Assay range:
0.125-10 ng/mL

20 µg /
100 µg of IgG

  
OK


OK


-


  
OK


OK


-


  
-


OK


OK


  
Suitable for urine and animal serum. Assay time: 2.5 hours.(KOG-200SE)

Suitable for human serum,tissue and cultured cells. Assay time: overnight +1.5 hours(KOG-HS10E)
Highly specific to 8-OHdG. Low cross reactivity to oxidized RNA(8-OHGua/8-OHG).
Anti Thymidine Glycol (TG) antibody
(Code: MTG-100P) Clone 2E8		 100 µg of IgG - - OK DNA-specific oxidative stress marker. Specific to TG in DNA polymer.
Lipid
oxidation
marker		 HEL (13HPODE-Lys adduct)
Hexanoyl-Lysine (HEL) ELISA kit
(Code: KHL-700E), ELISA: 450nm, 96 wells

Anti Hexanoyl-Lysine (HEL) antibody
(Code: MHL-021P) Clone 5F12		  
Assay range:
2 - 700 nmol/L

20 µg of IgG

  
OK


-

  
OK


-

  
OK


OK

 Nε-Hexanoyl-Lysine (HEL) is derived from 13-HPODE, and is a novel biomarker for early stage oxidation of n:6 polyunsaturated fatty acid. Detectable in urine, serum, tissue and cultured cells.
4-HNE
Anti 4-HNE monoclonal antibody (HNEJ2)
(Code: MHN-020P / MHN-100P)		  
20 µg /
100 µg of IgG		  
-
 		  
-
 		  
OK
 		 4-hydroxy-2-nonenal (4-HNE) is one of the major oxidized products of fatty acids. Specific to 4-HNE-modified proteins.
MDA
Anti MDA monoclonal antibody
(Code: MMD-030n) Clone 1F83

Malondialdehyde assay kit (TBARS)
(Code: KMD-008W) Colorimetric: 400-700nm scan		  
30 µg of IgG


Assay range:
0.08 - 10 µmol/L		  
-


OK
 		  
-


OK
 		  
OK


OK
 		 Specific to Malondialdehyde(MDA)-protein adducts such as dihydropyridine-lysine (DHP-lysine).
By utilizing 3rd derivative analysis, more reliable results than that by traditional TBARS assay.
Isoprostane
Urinary isoprostane ELISA
(Code: KIP-050) ELISA: 450nm, 96wells

Serum isoprostane ELISA
(Code: KIP-001W) ELISA: 450nm, 96wells		  
Assay range:
0.05 - 100 ng/mL

Assay range:
0.05 - 10 ng/mL		  
OK


-
 		  
-


OK
 		  
-


OK
 		  
15-Isoprostane F2t (also known as 8-epi-PGF2α or 8-iso-PGF2α) is produced by peroxidation of lipoproteins.
For serum or tissue samples, purification by C18/Silica Sep-Pak will be needed.
Anti Acrolein (ACR) antibody
(Code: MAR-020n / MAR-100n) Clone 5F6		 20 µg /
100 µg of IgG		 - - OK Specific to ACR modified proteins: formyl-dehydropiperidino-lysine (FDP-lysine).
Anti 4-hydroxy-2-hexenal (4-HHE) antibody
(Code: MHH-030n) Clone HHE53		 30 µg of IgG - - OK Specific to HHE-histidine Michael adduct (HHE-His).
Anti Crotonaldehyde (CRA) antibody
(Code: MCA-030n) Clone 82D3		 30 µg of IgG - - OK Specific to CRA-protein aduucts.
Anti Methylglyoxal (MG) antibody
(Code: MMG-030n) Clone 3C		 30 µg of IgG - - OK Specific to MG-protein adduct (argpyrimidine).
Anti 7-ketocholesterol (7-KC) antibody
(Code: MKC-020n) Clone 35A		 20 µg of IgG - - OK Biomarker of cholesterol oxidation. Applicable for frozen tissue only.
Mitochondria
Lipid peroxide probe (mitochondria) [NEW]
(Code: PLP-466J)		  
5 µg x3
 		  
-
 		  
-
 		  
OK
 		 Fluorescence probe for lipid peroxides specific to mitochondia. Suitable for cultured cells.
Lipid peroxide probe (DPPP) [NEW]
(Code: PLP-350J)		 10 mg - OK OK Fluorescence probe for lipid peroxides. For HPLC application.
Protein
oxidation
marker		 Dityrosine (Tyr dimer)
Dityrosine (DT) ELISA [NEW]
(Code: KDT-010E), ELISA: 450nm, 96 wells

Anti Dityrosine (DT) antibody
(Code: MDT-020P) Clone 1C3		  
Assay range:
0.05-12 µmol/L

20 µg of IgG
 		  
OK


-
 		  
OK


-
 		  
-


OK
 		  
New biomarker specific for protein oxidation. Assay time: overnight + 1.5hours.
Anti Dibromo-Tyrosine (DiBrY) antibody
(Code: MBY-020P) Clone 3A5		 20 µg of IgG - - OK Inflammation-related protein oxidation marker.
NO-modification
Anti Nitrotryptophan (NW) antibody [NEW]
(Code: MNW-020P) Clone 117C		  
20 µg of IgG
 		  
-
 		  
-
 		  
OK
 		 A novel nitrosative stress marker derived from peroxynitrite and tryptophan.
Nitrotyrosine ELISA
(Code: KNT-002W) 96 wells		 2 - 1500 nmol/L - OK Δ  
Anti-
oxidant
assay		 Total Antioxidant
Test kit for Potential Antioxidant(PAO), 96 wells
(Code: KPA-050) Colorimetric: 480-492 nm

TAC Peroxyl assay,  96 wells
(Code: KPR-005W) Luminescence		  
Assay range:
21.9- 4378 µmol/L

Assay range:
0.5～30 µmol/mL		  
-


-
 		  
OK


OK
 		  
OK


OK
 		  
Total antioxidant assay for serum, foods and beverages. Can detect both hydrophobic and hydrophilic antioxidants.
KPR-005W can detect peroxyl radicals(ROO·) scavenging activity.
GSH
Total Glutathione assay kit 100 tests [NEW]
(Code: KGS-419J) Colorimetric: 405-415 nm		  
Assay range:
5-100 µmol/L		  
-
 		  
-
 		  
OK
 		 The most abundant antioxidant in living cells. Applicable to a variety of samples such as plant tissues and bacteria.
Enzyme
assay		 SOD
SOD activity assay kit (WST-1 method)  [NEW]
(Code: KSD-311J) Colorimetric: 450 nm, 500 tests

SOD activity assay kit (Hematoxylin method)
(Code: KSD-005W) Colorimetric: 560 nm, 96 wells

Cu/Zn-SOD ELISA (Human SOD1)
(Code: KSE-040W) ELISA: 450 nm, 96 wells

Mn-SOD ELISA (Human SOD2)
(Code: KSG-025W) ELISA: 450 nm, 96 wells

ec-SOD ELISA (Human SOD3)
(Code: KSF-002W) ELISA: 450 nm, 96 wells		  



Assay range:
5-60 U/mL

Assay range:
0.04 - 5 ng/mL

Assay range:
25 - ng/mL

Assay range:
2 - 168 ng/mL		  
-


-


-


-


-
 		  
OK


Eryth-
rocyte

OK


-


-
 		  
OK


OK


OK


OK


OK
 		  
Can be applied to all animals, plants and bacteria.




SOD1 is mainly located in erythrocytes and tissue cells. Trace concentration of SOD1 would be detected in serum.
Mn-SOD(SOD2) is detectable in Mitochondria.
CAT
Catalase activity assay kit 96 wells
(Code: KCA-003W) UV: 240 nm		  
Assay range:
6-150 U/mL		  
-		  
Eryth-
rocyte		  
OK		  
MPO activity assay kit
(Code: KMP-008W) Colorimetric: 412 nm
96 microplate assays or 30 cubette assays		 Assay range:
8- 400 U/mL		 - OK OK  
GSH metabolism
Glutathion Peroxidase (GPx) assay kit 192 wells
(Code: KGX-005W) Colorimetric: 340 nm

Glutathion Reductase (GR) assay kit 192 wells
(Code: KGR-015W) Colorimetric: 340 nm		  
Assay range:
5-60 mU/mL

Assay range:
1.5-20 mU/mL		  
-


-
 		  
Blood


Blood
 		  
OK


OK
 		  
Redox
regulation
markers		 Thioredoxin
Thioredoxin I ELISA (human) 96 wells
(Code: KRX-004W) ELISA: 450 nm		  
Assay range:
0.4- 25 ng/mL		  
-
 		  
OK
 		  
Δ
 		  
Thioredoxin reductase I ELISA (human) 96 wells
(Code: KRR-004W) ELISA: 450 nm		 Assay range:
0.4- 25 ng/mL		 - OK Δ  
Peroxiredoxin I ELISA (human) 96 wells
(Code: KPX-005W) ELISA: 450 nm		 Assay range:
0.5 - 32 ng/mL		 - OK Δ  
Peroxiredoxin III ELISA (human) 96 wells
(Code: KPZ-002W) ELISA: 450 nm		 Assay range:
2 - 32 ng/mL		 - OK Δ  
Trace
elements
assay		 Fe
Iron assay kit (Ferrozine) 100 tests
(Code: CFE-005) Colorimetric: 540-580 nm

Iron assay kit (Nitroso-PSAP) 100 tests
(Code: CFE-010) Colorimetric: 740-760 nm

UIBC assay kit (Bathophenanthroline) 100 tests
(Code: CBC-800) Colorimetric: 540-550 nm		  
Assay range:
5-1,000 µg/dL

Assay range:
10-1,000 µg/dL

Assay range:
10-800 µg/dL		  
OK


OK


-
 		  
OK


OK


OK
 		  
OK


OK


-
 		  
Iron (Fe) is an important pro-oxidant factor which produce hydroxyl radical by fenton reaction. CFE-005 and CFE-010 can be also applied to extractes from animal tissue, plants and hair samples.

Detection of unsaturated iron binding capacity(UIBC). Species independent.
Cu
Copper assay kit (3,5-DiBr-PAESA) 100 tests
(Code: CCU-400) Colorimetric: 570-590 nm		  
Assay range:
3-400 µg/dL		  
OK
 		  
OK
 		  
OK
 		 Can detect total (free and ceruloplasmin - bond) copper. Also applicable to extractes from tissues, plants and hair.
Zn
Zink assay kit (5-Br-PAPS) 
(Code: CZN-001) Colorimetric: 550-580 nm		  
Assay range:
4-1,000 µg/dL		  
OK
 		  
OK
 		  
OK
 		 Can detect total (free and protein - bond) zinc. Also applicable to extractes from tissues, plants and hair.
Ca
Calcium assay kit (Chlorophosphonazo III)
(Code: CCA-020) Colorimetric: 680-700 nm, 200 tests

Calcium assay kit (o-Cresolphtalein-Complexone)
(Code: CCA-030) Colorimetric: 560-590 nm, 200 tests		  
Assay range:
0.05-20 mg/dL

Assay range:
0.2-30 mg/dL		  
OK


OK
 		  
OK


OK
 		  
OK


OK
 		 Applicable to serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples. Avoid EDTA containing samples.
Mg
Magnesium assay kit (Xyridil Blue-I) 100 tests
(Code: CMG-035) Colorimetric: 660 nm		  
Assay range:
0.05-3.5 mg/dL		  
OK
 		  
OK
 		  
OK
 		 Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium.

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