Tranilast, an orally active antiallergic compound, inhibits extracellular matrix production in human uterine leiomyoma and myometrial cells.
Fertil Steril. 2014 Aug;102(2):597-606.
Islam MS1, Protic O2, Ciavattini A3, Giannubilo SR3, Tranquilli AL3, Catherino WH4, Castellucci M2, Ciarmela P5.
- 1Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, Bangladesh.
- 2Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy.
- 3Department of Clinical Science, Polytechnic University of Marche, Ancona, Italy.
- 4Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
- 5Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy; Department of Information Engineering, Polytechnic University of Marche, Ancona, Italy. Electronic address: [email protected]
OBJECTIVE: To determine the effect of tranilast (an antiallergic drug known to suppress fibrosis or to stabilize mast cells) on extracellular matrix production in human leiomyoma and myometrial cells.
DESIGN: Laboratory study.
SETTING: University-affiliated laboratory.
PATIENT(S): Seven premenopausal women who were admitted to the hospital for myomectomy or hysterectomy.
INTERVENTION(S): Cells were treated with tranilast (300 μM) for 48 hours to measure extracellular matrix and activin-A expression by real-time reverse-transcription polymerase chain reaction and/or immunocytochemistry.
MAIN OUTCOME MEASURE(S): The expression of fibronectin, collagen1A1, versican, and activin-A in myometrial and leiomyoma cells.
RESULT(S): Tranilast decreased fibronectin, collagen 1A1, and versican messenger RNA (mRNA) expression in human primary leiomyoma cell culture. Similar results were found in an immortalized human leiomyoma cell line. Tranilast also decreased the mRNA expression of fibronectin, collagen 1A1, and versican in human primary myometrial cells. The reduced expression of fibronectin and collagen 1 were observed by immunocytochemistry as well. Tranilast also reduced profibrotic growth factor, activin-A mRNA expression in primary myometrial and leiomyoma cells.
CONCLUSION(S): Our results indicate that tranilast reduced fibronectin, collagen 1A1, versican, and activin-A expression in leiomyoma and myometrial cells, demonstrating its potential as an antifibrotic therapy for human leiomyomas.
KEYWORDS: Fibroid; activin-A; collagen 1A1; fibronectin; tranilast