Pathomorphosis  of experimental infection in mice infected with Streptococcus pneumonia, under the influence of immunotropic drugs

Bulletin of Experimental  Biology and Medicine: 2013, 155(4): 471-478.

Larisa M. Somova, Nadejda M. Kondrashova, Natalia G. Plekhova, Elena I. Drobot, Irina N. Lyapun

Laboratory of Cellular Biology and histopathology, G.P. Somov  Research Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences, 690087, Selskaya 1,  Vladivostok, Russia.



It is known that the pathogenesis of infection closely associated with the reactivity of the lymphoid organs, which provides the desired level of specific immunity and  activates nonspecific factors  of antimicrobial resistance [1, 2].  In this paper we studied the morphogenesis of experimental pneumonia caused by Streptococcus pneumoniae, under the influence of immune preparations to justify their  efficiency in community-acquired pneumonia. This question is important because immune failure often develops during the course of this severe disease. It was determined the positive dynamics of the resolution of the pathological process in lung of  animals, treated  by immunotropic drugs, compared with intact and  untreated  control  groups.  The picture of lymphoid organs testified to the activation of immune protection under the influence of used  drugs.



The studies carried out on model of adult  white mice infected intranasally  with St. pneumoniae 50 %  lethal dose (500 thousand bacterial cells). Animals were divided into 6 groups: group 1 – control 1, uninfected mice; group 2 –  control 2, animals infected  with  St. pneumoniae;  group 3 – infected animals treated with antibiotic moxifloksatsin; group 4 – infected animals treated with antibiotic and Likopid;  group 5- infected animals treated with antibiotic and tinrostim;  group 6 – infected animals treated with antibiotic and ronkoleukin. Histopathological picture of animal organs  (lung, spleen, thymus) treated with immunotpopic  drugs on the background antibiotic therapy, compared with those in animals treated with antibiotics alone (group 3).

The results of our experimental  investigations determined the pathomorphosis  of pneumonia, caused by St. pneumoniae, and leveling immunodeficiency states under the influence of immune preparations.   It was shown the clear advantage  of tinrostim  with respect to tissue manifestations  of reactivity cells of innate immunity (Figure). Our results substantiate the feasibility of tinrostim to accelerate the resolution of the inflammatory process, since this biopolymer is a potent stimulator of the functional activity of phagocytic cells [3]. In conjunction with Likopid and / or ronkoleukine, tinrostim can be especially recommended for inclusion in the base rate (antibacterial) therapy in patients with lingering pneumonia.


Figure: Pathomorphosis of  changes in lung and spleen of animals infected with St. pneumoniae and treated  by tinrostim:

a) hemorrhagic lung impregnation (the  red hepatization step) at untreated pneumonia (2 group, 3 days), x100; b) alveoli filled with polymorphic cells (the  grey hepatization step) at pneumonia treated by  antibiotic (3 group, 7th days postinfection, 5th days after the beginning of treatment), x100; c) active phagocytic reaction to background hemorrhagic lung edema by tinrostim applying  (5 group, 5th days postinfection, second days after the tinrostim applying), x100; d – perivascular infiltrate in the lung consisting of mononuclear cells 5 group, 14th days postinfection,  11 days after the tinrostim applying), x200; e – depletion of lymphoid tissue in  the spleen of deceased mice (2 group, 3 days postinfection), x100; f – macrophage-associated and lymphoid hyperplasia of the spleen when tinrostim applying (5 group, 14 days postinfection, 11 days tinrostim applying), x100. Hematoxylin and eosin coloring.



1. Avtandilov G.G., Barsukov V.S. Systematic study of immune and endocrine organs in infectious process. Archives of Pathology. – 1993. – № 1. – S. 7-12.

2. Abraham Е., Matthay M.A., Dinarello С.А. et al. Consensus conference definitions for sepsis, septic shock, acute lung injury, and acute respiratory distress syndrome: time for a reevaluation. Critical Care Med. 2000. – Vol. 28. – P. 232-235.

3. Zaporozhets T.S.,  Besednova N.N. Immunoactive biopolymers from marine aquatic organisms. Vladivostok, 2007. 218 p.

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